SEROLOGICAL ASSAY OF CULTURE FILTRATES FOR STAPHYLOCOCCUS ENTEROTOXIN
نویسندگان
چکیده
منابع مشابه
Serological Assay of Culture Filtrates for Staphylococcus Enterotoxin.
color from ammonia was produced by an additional 5 min of incubation at 37 C. All strains of Proteus, except those of P. inconstans, generated detectable amounts of ammonia (Table 1). None of the Escherichia organisms was urease-positive. These findings were identical with those yielded by the urease technique of Stuart et al. The sensitivity of the Berthelot color reaction permits detection of...
متن کاملRapid quantitative serological assay of staphylococcal enterotoxin B.
A simple, rapid method, based on the Oudin single diffusion technique, is described for the quantitative assay of staphylococcal enterotoxin B. The method yields reproducible results without close control of such assay variables as temperature, antiserum dilution, and assay time, provided that the ionic strength is maintained above 0.2 n sodium chloride equivalent.
متن کاملIdentification of the classical enterotoxin genes of Staphylococcus aureus in various foods by multiplex PCR assay
Background: An annual update of information about the prevalence of Staphylococcus aureus and staphylococcal enterotoxins (SEs) genes is required in every geographic area. Aims: This study was conducted to investigate the prevalence of the bacterium and type of associated enterotoxin genes in different food samples, using multiplex polymerase ...
متن کاملAn Improved Diffusion Assay for Quantifying the Polygalacturonase Content of Erwinia Culture Filtrates
Taylor, R. J., and Secor, G. A. 1988. An improved diffusion assay for quantifying the polygalacturonase content of Erwinia culture filtrates. Phytopathology 78:1101-1103. The agar diffusion procedure for quantifying pectolytic enzyme activity punched in the agarose with a # 1 cork borer. After incubation at 37 C for 17 was modified to optimize assay sensitivity and simplify its implementation. ...
متن کاملInhibition of immune hemolysis: serological assay for the heat-labile enterotoxin of Excherichia coli.
Sheep erythrocytes sensitized by incubation with the heat-labile enterotoxin (LT) of Escherichia coli are hemolyzed in the presence of anti-LT antiserum and complement. The Microtiter (Cooke Laboratory Products) technique was used to titrate anti-LT antibody in serum by this immune hemolysis reaction. Immune hemolysis was inhibited by preexposure of the anti-LT antiserum to soluble LT before ad...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1963
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.85.4.955-956.1963